Over-expression and purification of a pea mitochondrial heat-shock protein
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Over-expression and purification of a pea mitochondrial heat-shock protein by Alice Copsey

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Published .
Written in English


Book details:

Edition Notes

StatementAlice Copsey.
SeriesSussex theses ; S 5663
The Physical Object
Pagination141 leaves :
Number of Pages141
ID Numbers
Open LibraryOL16166252M

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Plasmodium falciparum heat shock protein 70 (PfHsp) is thought to play an essential role in parasite survival and virulence in the human host, making it a potential antimalarial drug target.   Over-expression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance. Biol Plant. ;54(1)– Biol Plant. ;54(1)– CrossRef Google ScholarCited by: 2.   The duplication and divergence of heat stress (HS) response genes might help plants adapt to varied HS conditions, but little is known on the topic. Here, we examined the evolution and function of Arabidopsis (Arabidopsis thaliana) mitochondrial GrpE (Mge) proteins. GrpE acts as a nucleotide-exchange factor in the Hsp70/DnaK chaperone machinery. Genomic data show that Cited by:   The heat shock protein gene promoter consensus sequence is the palindromic sequence TTCNNGAA (Santos et al. ). Finally, the Y-box motif has consensus sequence GATTGG and mediates redox-dependent transcription activation (Guan and Scandalios ). The seven promoter regions were examined for these four consensus by:

  Metabolic defense mechanisms: limiting ROS-mediated damage. Plant cells respond defensively to oxidative stress by removing the ROS and maintaining antioxidant defense compounds at levels that reflect ambient environmental conditions (Scandalios ).Metabolic containment mechanisms for ROS involving antioxidant genes and associated processes are likely to have Cited by:   Vacca RA, de Pinto MC, Valenti D, Passarella S, Marra E, de Gara L () Production of reactive oxygen species, alteration of cytosolic ascorbate peroxidase, and impairment of mitochondrial metabolism are early events in heat shock-induced programmed cell Cited by: 3.   Integrity of biomembranes is vital to living organisms. In bacteria, PspA is considered to act as repairing damaged membrane by forming large supercomplexes in Arabidopsis (Arabidopsis thaliana). Vulnerable to oxidative stress, photosynthetic organisms also contain a PspA ortholog called VIPP1, which has an additional C-terminal tail ([Vc][1]).Cited by: Arabidopsis thaliana seeds without functional SEED MATURATION PROTEIN1 (SMP1), a boiling soluble protein predicted to be of intrinsic disorder, presumed to be a LATE EMBRYOGENESIS ABUNDANT (LEA) family protein based on sequence homology, do not enter secondary dormancy after 3 days at 40 °C. We hypothesized that SMP1 may protect a heat labile protein involved in the promotion of Cited by: 8.

Purification of a heterodimeric betaine aldehyde dehydrogenase from wild amaranth plants subjected to water deficit. Biochem. Biophys. Res. Commun. (). Fils-Lycaon BR, Wiersma PA, Eastwell KC, Sautiere P. A cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like. To reveal the mechanism of salinity stress alleviation by arbuscular mycorrhizal fungi (AMF), we investigated the growth parameter, soluble sugar, soluble protein, and protein abundance pattern of E. angustifolia seedlings that were cultured under salinity stress ( mmol/L NaCl) and inoculated by Rhizophagus irregularis (RI). Furthermore, a label-free quantitative proteomics approach was Cited by: 1. In nature, C. reinhardtii is a soil organism, but it can be grown in the laboratory either in liquid culture or on agar in simple mineral salts, and many of the recipes proposed for algae in general will suffice for its culture (see Bold, , and Watanabe, ). Table gives the composition of the media most often utilized in current Chlamydomonas research, and recipes for their. Full text of "Molecular biology and biotechnology of plant organelles: chloroplasts and mitochondria" See other formats.